Journal: PLoS ONE

Article Title: HDL Induces the Expression of the M2 Macrophage Markers Arginase 1 and Fizz-1 in a STAT6-Dependent Process

doi: 10.1371/journal.pone.0074676

Figure Lengend Snippet: BMDM were grown as previously described and stimulated for 15min with either HDL alone (50μg/mL), IL-4 alone (10ng/mL) or both. Phosphorylation state for STAT6 (A) and STAT3 (C) was assessed by western blot. As a positive control for STAT3 activation, cells were treated with IL-10 (10ng/mL) for 30 min. Signal quantification for phospho-STAT6 (B) is the result of three independent experiments. Asterisks indicate statistically significant difference, either compared to control, or between 2 conditions when linked by a bar (*p<0.05, **p<0.01).

Article Snippet: The following antibodies were used: Phospho-STAT3 (Tyr705) and total-STAT3 (Cell Signaling), Phospho-STAT6 (Tyr641) (Imgenex), STAT6 (Santa Cruz Biotechnology Inc.), and GAPDH (Millipore).

Techniques: Phospho-proteomics, Western Blot, Positive Control, Activation Assay, Control

Journal: PLoS ONE

Article Title: HDL Induces the Expression of the M2 Macrophage Markers Arginase 1 and Fizz-1 in a STAT6-Dependent Process

doi: 10.1371/journal.pone.0074676

Figure Lengend Snippet: BMDM were isolated from STAT6 +/+ (black bars) and STAT6-/- (hatched bars) mice and were stimulated for 6h with HDL (50μg/mL) or IL-4 (10ng/mL). The induction of Arg-1 or Fizz-1 mRNA in the STAT6+/+ cells by HDL was ~3X for each and by IL-4, ~1800 or 3300, respectively; these values were set to 100%. Results are representative of three independent experiments. Asterisks indicate statistically significant differences compared to corresponding STAT6 +/+ conditions (***p<0.001).

Article Snippet: The following antibodies were used: Phospho-STAT3 (Tyr705) and total-STAT3 (Cell Signaling), Phospho-STAT6 (Tyr641) (Imgenex), STAT6 (Santa Cruz Biotechnology Inc.), and GAPDH (Millipore).

Techniques: Isolation

Journal: Cell reports

Article Title: Mass Cytometry Reveals Global Immune Remodeling with Multi-lineage Hypersensitivity to Type I Interferon in Down Syndrome

doi: 10.1016/j.celrep.2019.10.038

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit monoclonal anti-human phospho-STAT6 (Tyr641) (clone 18) , Fluidigm , Cat#3168012A; RRID:AB_2811103.

Techniques: Recombinant, Mass Cytometry, Software